Brief Title
Validation of DNA Methylation Biomarkers for Oral Cancer Detection-Follow up Study
Official Title
Validation of DNA Methylation Biomarkers for Oral Cancer Detection-Follow up Study
Brief Summary
This is a follow up study which aims to evaluate the correlation between several methylated genes (potential biomarkers) and oral cancers. A prospective case control trial is designed with sample size of at least 300 cases with estimated 200 subjects with precancerous lesion or oral cancer, and 100 subjects with normal oral mucosa. This study is approved by the National Taiwan University Hospital Research Ethics Committee. After signing the informed consent, all subjects will receive an intraoral examination and followed by epithelial cells collection with oral swab. The gDNA will be extracted from the oral swab collected cells and followed by bisulfite conversion procedures. Subsequently, bisulfite converted DNA will be subjected to methylated gene detection by Real-Time PCR. The methylation index (clinical sensitivity and specificity) of oral cancer related genes will be evaluated. For diagnosis confirmation, photos and biopsy specimens will be taken upon observation of abnormal lesion.
Study Type
Observational
Primary Outcome
DNA methylation
Condition
Oral Cancer
Study Arms / Comparison Groups
normal oral mucosa
Description:
Publications
* Includes publications given by the data provider as well as publications identified by National Clinical Trials Identifier (NCT ID) in Medline.
Recruitment Information
Estimated Enrollment
300
Start Date
April 18, 2016
Primary Completion Date
April 17, 2019
Eligibility Criteria
Inclusion Criteria: - Subject with age over 20. - Subjects with oral mucosal findings (normal, oral mucosal lichen planus, precancerous lesion, and oral cancer) or subjects who have involved in the previous study (NCT01945697). - Subjects agrees to sign the informed consent form. Exclusion Criteria: - Pregnant woman. - Subject disagrees to sign the informed consent form.
Gender
All
Ages
20 Years - N/A
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Contacts
, 886-2-23123456, [email protected]
Location Countries
Taiwan
Location Countries
Taiwan
Administrative Informations
NCT ID
NCT02902406
Organization ID
201601034RINB
Responsible Party
Sponsor
Study Sponsor
National Taiwan University Hospital
Study Sponsor
, ,
Verification Date
September 2016