Cigarette Smoking and Oral Microbiota

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Brief Title

Cigarette Smoking and Oral Microbiota

Official Title

The Effect of Smoking on Oral Microbiota

Brief Summary

      Background:

      - Normal bacteria and other tiny organisms (the microbiota) live in the mouth and nose. They
      contribute to human health in many ways, including digesting food and balancing hormones.
      Testing samples from the mouth can show how microbiotas are related to health and disease.
      However, the microbiota in a person's mouth differs depending on the methods of collection
      and the part of the mouth that is tested. Understanding what can change the microbiota
      (including mouth sites, and what a person eats or smokes) will give more information on how
      to study oral microbiota and smoking-related cancers and other diseases.

      Objectives:

        -  To see how smoking affects the microbiotas in mouth and nose.

        -  To determine which collection method for mouth specimens should be used for studying
           microbiota.

      Eligibility:

        -  Individuals at least 18 years of age who have been using tobacco products regularly for
           at least 5 years.

        -  Individuals at least 18 years of age who have never smoked.

      Design:

        -  Participants will be screened with a physical exam and medical history.

        -  Participants will have a dental exam. They will provide a saliva sample. The dentist
           will take swabs from the inside of the mouth, including the tongue, tonsils, gums, and
           teeth. The inside of the nose will also be swabbed.

        -  Participants will also fill out a questionnaire. It will ask about their history of
           smoking and consumption of alcohol, tea, and coffee. It will also ask about current
           medications, including antibiotics.
    

Detailed Description

      The oral microbial community (the microbiota), is centrally related to nutrition, metabolism,
      immunity, inflammation, and endocrine balance. Cigarette smoking is associated with serious
      health outcomes including cancer, cardiovascular disease and chronic lung disease. However,
      little is known about the relationship between cigarette smoking, oral microbiota and
      tobacco-related health outcomes. Microbiotas in the different sites of the same oral cavity
      vary widely. Recent studies indicated that the difference in microbial profiles between
      buccal mucosa and gingival plaque is as distinct as the difference between tongue and stool.
      To date, knowledge about the effect of smoking on oral microbiotas is lacking. As an initial
      step toward understanding the role of the oral microbiota in smoking-related health outcomes,
      we propose a pilot study to evaluate the effects of cigarette smoking on the microbiotas in
      buccal rinse obtained from the oral cavity and across 8 different oral cavity sites/niches
      (saliva, swabs from tongue dorsum, hard palate, buccal mucosa, keratinized gingiva, palatine
      tonsils, supragingival plaque, subgingival plaque). We will seek to recruit up to 50
      volunteers through the Eastman Dental General Dentistry Clinic, Rochester, NY, including 25
      current smokers with > 5 years of smoking history and 25 never smokers as a comparison group.
      Age-group, gender, and race (Caucasian/African- American) will be matched for the two groups.
      In each subject, the buccal cell (mouthwash) collection will be collected according to the
      PLCO buccal cell collection protocol. In addition, collections across 8 different oral cavity
      sites will be done using a protocol developed for the Human Microbiome Project. These 9
      collections will be used for high throughput sequencing of 16S rRNA microbial genes at the
      Institute of Genome Sciences, University of Maryland School of Medicine. The findings will
      provide information about the effect of smoking on microbial taxa composition and relative
      abundance at each collection site. This pilot study will demonstrate the effects of smoking
      on oral microbiotas across different oral cavity sites and will also demonstrate which oral
      collection site is most promising for the detailed study about cigarette smoking and oral
      microbiota. In addition, the data collected will provide a validation framework to use the
      PLCO buccal specimens for large-scale studies. The information about the validity of using
      PLCO buccal cell collection will be an invaluable asset for further large scale studies of
      the microbiota and cancer risk. We will also be able to investigate if there is any taxonomy
      or microbial diversity difference between heavy smokers and never-smokers. This pilot study
      will provide essential information about collection, design and analyses that will enable
      further studies of the relationships between cigarette smoking, the oral microbiome and
      tobacco-related cancers.

      Under an Amendment, the participants will be contacted to provide another set of oral swabs
      and mouthwash samples. The goal is to examine the stability or oral microbiota in 3-6 months
      and confirm the results based on the first time point. The collection methods, assays and
      analyses will be analogous to those performed previously.
    


Study Type

Observational


Primary Outcome

This is a pilot study to demonstrate the effects of smoking on oralmicrobiotas across differenct oral cavity sites


Condition

Microbiota


Study Arms / Comparison Groups

 cases
Description:  smokers

Publications

* Includes publications given by the data provider as well as publications identified by National Clinical Trials Identifier (NCT ID) in Medline.

Recruitment Information



Estimated Enrollment

50

Start Date

May 23, 2013

Completion Date

June 26, 2020

Primary Completion Date

December 5, 2014

Eligibility Criteria

        -  INCLUSION CRITERIA

        This study will recruit a convenience sample of 50 volunteers (25 current smokers with at
        least 5 years of smoking history, 25 never smokers). Current smokers are defined as
        individuals who have smoked more than 100 cigarettes in their lifetime and have smoked 5 or
        more cigarettes in the last 24 hours. Recent use of other tobacco products (pipe, cigar,
        snuff, cigarillos, and chewing tobacco) is an overall exclusion, but use in the remote past
        (> 6 month ago) is acceptable in smokers. Never smokers are defined as individuals who have
        never smoked cigarettes nor used any other tobacco products including pipe, cigar, snuff,
        cigarillos, or chewing tobacco.

        The ethnic mix of the clinic is roughly 50% Caucasians and 50% African- Americans with a
        small number of unspecified or other racial groups. The median age is about 50 and the
        gender mix consists of an equal number of men and women. We therefore will select smokers
        and frequency match to non-smokers based on ethnicity (White, African-American), gender
        (male, female), and age (above or below the median, estimated to be 50).

        EXCLUSION CRITERIA

        We will exclude pregnant women and other racial groups because they may represent very
        small numbers and thus be difficult to match. Hormonal changes associated with pregnancy
        and unique cultural habits associated with specific ethnic groups could be associated with
        highly unique or variable microbiome patterns, and therefore reduce the power to detect
        differences associated with smoking which is our primary goal. We will also exclude
        subjects with antibiotic usage in the last three months and subjects with previous
        diagnosed major periodontal disease or cancer because they might be potential confounders.
      

Gender

All

Ages

25 Years - 80 Years

Accepts Healthy Volunteers

Accepts Healthy Volunteers

Contacts

Neil E Caporaso, M.D., , 

Location Countries

United States

Location Countries

United States

Administrative Informations


NCT ID

NCT01862809

Organization ID

999913103

Secondary IDs

13-C-N103

Responsible Party

Sponsor

Study Sponsor

National Cancer Institute (NCI)


Study Sponsor

Neil E Caporaso, M.D., Principal Investigator, National Cancer Institute (NCI)


Verification Date

June 2020