Tzanck Smear With Methylene Blue Stain for Herpes

Brief Title

Tzanck Smear With Methylene Blue Stain for Herpes

Official Title

A Comparative Study of Tzanck Smear With Methylene Blue Versus Giemsa Stain to Diagnose Herpes Simplex, Herpes Zoster and Varicella Zoster Skin Infections

Brief Summary

      To compare the sensitivity of Tzanck smear with methylene blue stain versus traditional
      Giemsa stain in patients with herpes infection.
    

Detailed Description

      A smear from the lesional skin of the patients suspected having Herpes simplex, Herpes zoster
      and Varicella zoster skin infections will be collected (in a clinical routine setting). The
      specimen will be stained with methylene blue in addition to the Giemsa stain. Polymerase
      chain reaction study for herpes antigen will be performed in some of the specimens. The
      sensitivity, specificity, positive predictive value and negative predictive value will be
      calculated from the results of the study.
    

Study Phase

Phase 3

Study Type

Interventional


Primary Outcome

Sensitivity

Secondary Outcome

 Factors that contribute the results from Methylene blue staining

Condition

Herpes Simplex

Intervention

Methylene blue

Study Arms / Comparison Groups

 Case
Description:  Patients who clinically diagnosed as herpes simplex, herpes zoster or varicella zoster skin infection.

Publications

* Includes publications given by the data provider as well as publications identified by National Clinical Trials Identifier (NCT ID) in Medline.

Recruitment Information


Recruitment Status

Diagnostic Test

Estimated Enrollment

150

Start Date

March 7, 2017

Completion Date

March 6, 2018

Primary Completion Date

December 7, 2017

Eligibility Criteria

        Inclusion Criteria:

          -  patients with clinically suspected of having herpes simplex, herpes zoster, varicella
             zoster skin infections.

          -  Those who have vesicular skin lesion such as acute eczema, Paederus dermatitis.

          -  Those are well cooperative.

          -  Age > 18 years old.

        Exclusion Criteria:

          -  Those who are not willing to have specimen from skin lesions to be collected.
      

Gender

All

Ages

18 Years - N/A

Accepts Healthy Volunteers

No

Contacts

Pawinee Rerknimitr, MD, MSc, +6622564253, [email protected]

Location Countries

Thailand

Location Countries

Thailand

Administrative Informations


NCT ID

NCT03178747

Organization ID

674/59


Responsible Party

Principal Investigator

Study Sponsor

Chulalongkorn University

Collaborators

 Dermatological Society of Thailand

Study Sponsor

Pawinee Rerknimitr, MD, MSc, Principal Investigator, Chulalongkorn University


Verification Date

August 2017