Effect of Pioglitazone Administered to Patients With Adrenomyeloneuropathy

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Brief Title

Effect of Pioglitazone Administered to Patients With Adrenomyeloneuropathy

Official Title

Effect of Pioglitazone Administered to Patients With Adrenomyeloneuropathy: a Phase II, Single-arm, Multicentric Clinical Trial

Brief Summary

      X-linked adrenoleukodystrophy is a rare, demyelinating and neurodegenerative disorder, due to
      loss of function of a fatty acid transporter, the peroxisomal ABCD1 protein. Its more
      frequent phenotype, the adrenomyeloneuropathy in adults, is characterized by axonal
      degeneration in spinal cord, spastic paraparesis and a disabling peripheral neuropathy.
      Actually, there is no efficient treatment for the disease. The work of the researchers in the
      last twelve years dissecting the physiopathological basis of the disorder has uncovered an
      involvement of the early oxidative stress in the neurodegenerative cascade and mitocondrial
      depletion. In a preclinical trial they have observed that pioglitazone, a PPARγ/PGC-1α axis
      metabolic activator with immunomodulatory, anti-inflammatory and antioxidant response
      regulator properties, efficiently reverse the clinical symptoms and the axonal degeneration
      in the mouse model for the disease and normalize stress and mitochondrial depletion

      The researchers will test the effectiveness of the drug in terms of motor function and
      correction of oxidative damage markers in proteins and DNA and inflammation markers in an
      open trial. Fifteen-twenty patients will be included and clinically explored and assessed in
      the HU of Bellvitge and the HU of Donostia using clinical scales for spasticity, evoked
      potentials, electroneurinograms and cranial RMN. The information will be collected in a data
      base that will be of great value to improve the present attention and the future follow-up of
      the patients and to facilitate their inclusion in therapeutic randomized, double blind,
      against placebo, multicentric and international clinical trials.

Detailed Description

      Proof of concept for this trial is provided by the results of biochemical, neuropathological
      and motor effects of pioglitazone in two mouse models of AMN. Pioglitazone was given orally
      (9 mg/kg/day) for two months in both models.

      The Abcd1-null mouse model already shows at 3,5 months biochemical signs oxidative stress
      that increase with time and are then associated with energy homeostasis alterations, although
      first clinical signs of AMN-i.e. axonopathy and locomotor impairment-appear at 20 months. In
      these mice, there are mitochondrial anomalies, decreased levels of PGC-1α which is a master
      regulator of mitochondrial biogenesis, and decreased levels and activity of SIRT1α, which
      activates PGC-1α.

      The Abcd1-null mouse can be considered as a "AMN-like" model, because of the absence of
      demyelinating lesions in brain and spinal cord, the presence of non-inflammatory
      ''dying-back'' axonopathy in peripheral nerves and spinal cord and its late-onset motor
      deficits that all are hallmarks of AMN in X-ALD patients. This model was used to assess the
      efficacy of pioglitazone on several biochemical markers in the spinal cord of Abcd1-null mice
      (N=12), using comparisons with placebo-¬treated Abcd1-null mice (N=12) or wild-type mice

      In Abcd1-null mice treated with pioglitazone at 10,5 months of age and studied at 12 months
      (1,5 months following the beginning of the ongoing treatment), mitochondrial anomalies were
      corrected to the level of wild type control mice. Indeed, mitochondrial DNA and protein
      (including PGC-1α, NRF1 and TFAM) levels were corrected; as well as mitochondrial metabolism,
      as assessed by pyruvate kinase activity, ATP and NAD+ concentrations. Pioglitazone had no
      effect on SIRT1 expression (mRNA and protein levels). However, pioglitazone significantly
      lowered the carbonylation of SIRT1 protein, which presumably accounts for the observed rescue
      of SIRT1 activity.

      In these mice treated with pioglitazone, oxidative lesions in the spinal cord were reversed.
      Studied oxidative stress biomarkers included markers of oxidative lesions to proteins (GSA,
      AASA), lipids (MDAL) and carbohydrates (CEL). Additionally, the activity and concentration
      level of antioxidant enzymes GPX1, which were increased in untreated Abcd1-null mice, but not
      SOD2, was normalized to the level of wild type mice.

      The second mouse model is the double knockouts (DKO) in which both Abcd1 and Abcd2
      transporters are inactivated. The Abcd1-/Abcd2-/-DKO exhibits greater VLCFA accumulation in
      spinal cord (Pujol et al., 2004), higher levels of oxidative damage to proteins, and a more
      severe AMN-¬like pathology, with earlier onset of motor impairment than the single Abcd1-null
      mouse (12 months in the DKO compared to 20 months in Abcd1-null mice). Efficacy of
      pioglitazone at the motor and neuropathologic levels was studied in 17 Abcd1-/Abcd2-/-mice
      comparing them with placebo-treated Abcd1-/Abcd2-/-mice (N=17) and wild-type mice (N=25).

      In Abcd1-/Abcd2-/-mice treated with pioglitazone at 13 months of age and studied at 15 or 17
      months (treatment duration of 2 to 4 months), axonal degeneration was prevented, as shown by
      the normalization to the control level of number of APP or synaptophysin positive axons.

      Also, pioglitazone arrested the progression of locomotor deficits in these mice, as assessed
      by the treadmill test and the bar-cross test. Indeed, the locomotor performances of
      pioglitazone DKO after four months of treatment mice reached the performances of the

      Overall, these studies show the efficacy of treatment with pioglitazone in "AMN-like mice
      "and provide a strong rationale for conducting a preliminary open clinical trial with
      pioglitazone in AMN patients.

Study Phase

Phase 2

Study Type


Primary Outcome

2 Minute Walk Test (2MWT)

Secondary Outcome

 Timed Up and Go (TUG) test





Study Arms / Comparison Groups

Description:  Pioglitazone 15 mg tablets 2/day during 2 years


* Includes publications given by the data provider as well as publications identified by National Clinical Trials Identifier (NCT ID) in Medline.

Recruitment Information

Recruitment Status


Estimated Enrollment


Start Date

January 2016

Completion Date

July 2019

Primary Completion Date

March 2019

Eligibility Criteria

        Inclusion Criteria:

          -  Clinical signs of AMN with at least pyramidal signs in the lower limbs and
             difficulties to run.

          -  Presence of motor deficit according to the EDSS scale

          -  Ability to perform the 2MWT

          -  Normal brain MRI or brain MRI showing abnormalities that can be observed in AMN
             patients without cerebral form of X-ALD with a maximum Loes score of 4

          -  Ejection fraction > 50% at echocardiogram

          -  Normal electrocardiogram

          -  Normal urine cytology

          -  Normal liver function, as assessed by plasma ASAT, ALAT, PAL, γGT, bilirubin measures
             (≤2.5-fold normal values)

          -  Normal kidney function as assessed by plasma urea, creatinin (≤ 2-fold normal values)

          -  Appropriate steroid replacement if adrenal insufficiency is present

          -  Informed consent

          -  Affiliated to the Spanish Public Health System

        Exclusion Criteria:

          -  Gadolinium enhancement on T1 sequence of any abnormal hypersignal of white matter,
             including myelinated pyramidal tracts, visible at brain MRI on FLAIR sequences

          -  Brain MRI abnormalities of the "AMN type" with a Loes score > 4

          -  Any abnormal hypersignal of white matter visible on FLAIR sequences other than of "AMN
             type" and related to X-ALD

          -  Patients taking pioglitazone or another glitazone during the past 6 months

          -  Diabetic patients (type I or II)

          -  Fasting blood glucose > 125 mg/L

          -  Glycosylated hemoglobin > 6%

          -  History of heart failure

          -  Heart failure (NYHA III to IV) or ejection fraction ≤ 50%

          -  History of cardiac disease

          -  [Hemoglobin] < 13g/dl in males, <12 g/dl in women

          -  Absolute neutrophil count (ANC) <1500 cells/mm3

          -  Platelet count <100,000 cells/mm3

          -  Significant peripheral edema (2+ or more on the Assessment Chart for Pitting Edema) of
             the extremities of any etiology

          -  Any evolutive malignancy during the last five years

          -  Prior or current bladder cancer

          -  Smokers (one pack/ day or more for at least 20 years), current or former

          -  Women with history of osteoporosis

          -  Menopaused woman with T-score < -2.5 on osteodensitometry measurement

          -  Any evolutive medical disease other than AMN

          -  Any psychiatric disease

          -  Pregnant or breastfeeding woman

          -  Either no pre-menopaused woman or no menopaused woman not taking any contraceptive

          -  Hereditary intolerance to galatose, or malabsorption of glucose or galactose due the
             presence of monohydrated lactose.

          -  Hypersensibility to the active substance or to galactose (excipient)

          -  Concomitant treatment with cytochrome P450 CYP 2C8 inhibitors (e.g. gemfibrozil) or
             inducers (e.g. rifampicin)

          -  Taking of either vitamin A, E or lipoic acid during the past 3 months

          -  Contraindications for MRI procedure such as subjects with paramagnetic materials in
             the body, such as aneurysm clips, pacemakers, intraocular metal or cochlear implants

          -  Present participation to another therapeutic clinical trial for ALD

          -  Not easily contactable by the investigator in case of emergency or not capable to call
             the investigator

          -  Gross hematuria of unknown origin




18 Years - 65 Years

Accepts Healthy Volunteers



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Location Countries


Location Countries


Administrative Informations



Organization ID


Responsible Party


Study Sponsor

Onofre, Aurora Pujol, M.D.


 Instituto de Salud Carlos III

Study Sponsor

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Verification Date

March 2019