Brief Title
A Search for Helicobacter Pylori in Localized Vulvodynia
Official Title
A Laboratory and Clinical Search for the Presence of H. Pylori and Treatment Against it in Localized Vulvodynia (Vestibulitis, Vestibulodynia).
Brief Summary
Hypothesis: to examine a possible association between localized vulvodynia and H. pylori
infection.
Detailed Description
Subsequent sections from the paraffin blocks were prepared and stained by modified Giemsa.
Immunostaining for H. pylori was done as described. In short, tissue sections were
deparaffinized in xylene, rehydrated through decreasing concentrations of alcohol ending in
phosphate-buffered saline (PBS), and subjected to pretreatment with Proteinase K (8 minutes).
The sections were quenched with 3% hydrogen peroxidase, incubated with protein block for 15
minutes at room temperature, and washed in PBS. Tissues then were incubated with polyclonal
rabbit anti-H pylori antibody (dilution, 1:10; clone ch-20 429, DAKO, Carpinteria, CA).
Finally, sections were washed in 0.05% polysorbate 20 in PBS, pH 7.4, and the bound antibody
was detected using streptavidin and biotinylated secondary antibody with diaminobenzidine as
the chromogen. Sections were counterstained with hematoxylin, dehydrated, and mounted.
Negative controls were sections treated as above, but instead of incubation with the primary
antibody, they were incubated with 1% bovine serum albumin in PBS.
Vulvar biopsies of seven other women without localized vulvodynia served as healthy controls.
The positive and negative control gastric tissues for the immunohistochemical stain of the H.
pylori microorganisms were obtained from the archives of the Department of Pathology.
Study Type
Observational
Condition
Vulvodynia
Publications
* Includes publications given by the data provider as well as publications identified by National Clinical Trials Identifier (NCT ID) in Medline.
Recruitment Information
Estimated Enrollment
27
Start Date
May 2004
Completion Date
June 2007
Eligibility Criteria
Inclusion Criteria:
- Paraffin blocks of vestibular tissues from patients with Vestibulodynia
Exclusion Criteria:
- Patients without vestibulodynia
Gender
Female
Ages
18 Years - 50 Years
Accepts Healthy Volunteers
No
Contacts
Jacob Bornstein, MD, ,
Location Countries
Israel
Location Countries
Israel
Administrative Informations
NCT ID
NCT00501774
Organization ID
Sabo-1
Study Sponsor
Western Galilee Hospital-Nahariya
Study Sponsor
Jacob Bornstein, MD, Principal Investigator, westen Galilee Hospital
Verification Date
July 2007