A Search for Helicobacter Pylori in Localized Vulvodynia

Brief Title

A Search for Helicobacter Pylori in Localized Vulvodynia

Official Title

A Laboratory and Clinical Search for the Presence of H. Pylori and Treatment Against it in Localized Vulvodynia (Vestibulitis, Vestibulodynia).

Brief Summary

      Hypothesis: to examine a possible association between localized vulvodynia and H. pylori
      infection.
    

Detailed Description

      Subsequent sections from the paraffin blocks were prepared and stained by modified Giemsa.
      Immunostaining for H. pylori was done as described. In short, tissue sections were
      deparaffinized in xylene, rehydrated through decreasing concentrations of alcohol ending in
      phosphate-buffered saline (PBS), and subjected to pretreatment with Proteinase K (8 minutes).
      The sections were quenched with 3% hydrogen peroxidase, incubated with protein block for 15
      minutes at room temperature, and washed in PBS. Tissues then were incubated with polyclonal
      rabbit anti-H pylori antibody (dilution, 1:10; clone ch-20 429, DAKO, Carpinteria, CA).
      Finally, sections were washed in 0.05% polysorbate 20 in PBS, pH 7.4, and the bound antibody
      was detected using streptavidin and biotinylated secondary antibody with diaminobenzidine as
      the chromogen. Sections were counterstained with hematoxylin, dehydrated, and mounted.
      Negative controls were sections treated as above, but instead of incubation with the primary
      antibody, they were incubated with 1% bovine serum albumin in PBS.

      Vulvar biopsies of seven other women without localized vulvodynia served as healthy controls.

      The positive and negative control gastric tissues for the immunohistochemical stain of the H.
      pylori microorganisms were obtained from the archives of the Department of Pathology.
    


Study Type

Observational




Condition

Vulvodynia



Publications

* Includes publications given by the data provider as well as publications identified by National Clinical Trials Identifier (NCT ID) in Medline.

Recruitment Information



Estimated Enrollment

27

Start Date

May 2004

Completion Date

June 2007


Eligibility Criteria

        Inclusion Criteria:

          -  Paraffin blocks of vestibular tissues from patients with Vestibulodynia

        Exclusion Criteria:

          -  Patients without vestibulodynia
      

Gender

Female

Ages

18 Years - 50 Years

Accepts Healthy Volunteers

No

Contacts

Jacob Bornstein, MD, , 

Location Countries

Israel

Location Countries

Israel

Administrative Informations


NCT ID

NCT00501774

Organization ID

Sabo-1



Study Sponsor

Western Galilee Hospital-Nahariya


Study Sponsor

Jacob Bornstein, MD, Principal Investigator, westen Galilee Hospital


Verification Date

July 2007